Human Chromosomes: Principles and Techniques /

A state-of-the-art presentation of the technology for studying human chromosomes, this book serves as a basic techniques manual designed to present the current working protocols in cytogenetics. Beginning with a description of tissue culture procedures for all tissue types from blood to solid tumors, subsequent chapters provide extensive coverage of chromosome preparations, differential staining techniques, chromosome sorting, high-resolution banding, fragile X analysis, sister chromatoid exchanges and DNA replication. A new chapter entitled "Molecular cytogenetic techniques" covers the latest fluorescent in-site hybridization (FISH) procedures illustrated by 20 colour plates.
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