Receptors: Molecular Biology, Receptor Subclasses, Localization, and Ligand Design, Volume 12: Volume 12: Receptors - Molecular Biology, Receptor ... Design (Methods in Neurosciences) (Vol 12)

Receptors initiate the means by which cellular regulators exert their actions on targets. Because of the central role of cell-cell communication and signal transduction, receptors are of intrinsic interest to neuroscientists. Receptor studies utilize both traditional methods of analysis and modern molecular techniques.

Key Features
* Methods presented for easy adaptation to new systems
* Comprehensive protocols included for molecular techniques (PCR, cloning, transfection, coupling); techniques for the determination of receptor subclasses; techniques for localization (in situ hybridization, immunocytochemistry); ligand design (radioactive techniques, biotinylated techniques); receptor-associated kinase
* Methodology described for the following receptors: acetylcholine, angiotensin II, bombesin/GRP, dopamine, GABA, G protein-coupled receptors, neurotensin, NGF, NPY, serotonin, somatostatin, tachykinin