Protein Purification Methods: A Practical Approach (The Practical Approach Series)

The first book of a two volume treatise on protein purification methods and applications, this title covers both traditional and recently developed techniques for initial purification, which is fundamental to any investigation of protein chemistry. The theory and basic principles of each technique are discussed, including initial planning, clarification and extraction, concentration of the extract, structure-based separation, activity-based purification, and gel permeation chromatography. Problem areas and solutions are highlighted at each stage, making this book equally valuable to novice researchers and more experienced protein biochemists who will find it a reliable and readily accessible bench manual.