Molecular Plant Pathology: A Practical Approach Volume I (Practical Approach Series, 85)

Molecular plant pathology has directly benefited from advances in modern molecular techniques. These techniques have been applied both to pathogen and plant, enhancing our understanding of the organisms themselves and of the complex interactions which determine compatibility between them and their host plants. This new book and its companion volume represent the first comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Detailed protocols are included which address a wide range of investigations from plant pathogen isolation and culture, through physiology and biochemistry, to techniques for localizing genes and their products within the cells of the infected plants. Topics in Volume I include pathogens and pathogen manipulation, nucleic acid isolation and hybridization techniques, analysis of gene expression, and in situ hybridization. Researchers in plant science, molecular biology, and related areas will find the two volumes of Molecular Plant Pathology to be an invaluable experimental resource for this exciting and fast moving field, providing a wealth of easy-to-follow protocols supported by expert advice and guidance.