In Situ Hybridization: A Practical Approach

In Situ Hybridization: A Practical Approach is a comprehensive practical guide with step-by-step laboratory protocols for the preparation of cloned DNA, RNA, and oligonucleotide probes (with both radioactive and non-radioactive labels) and their hybridization to mRNA, viral DNA, and chromosomal DNA. Methods are provided for the analysis of cells, tissue sections, and whole embryos. In addition, techniques are presented for carrying out in situ hybridization in combination with the immunochemical detection of protein, and for using it at the electron microscopic level. This detailed work is essential for any researcher wishing to master the widespread applications of this important biotechnology.