HPLC: A Practical User's Guide

HPLC: A Practical User's Guide is essential for chromatographers needing a proven training manual for setting up and running an HPLC system, for optimizing and cleaning columns and separations, and for obtaining separations and maximizing information from their system.

Relying upon 15 years of laboratory and field troubleshooting experience, the author explains the basic techniques and shows how to use them to optimize separations. Proven methods are provided for starting up and maintaining HPLC systems, cleaning and troubleshooting column to the system problems, and systematic column treatment and methods development, both manual and automated.

Coverage includes preparative HPLC, Diode Array, UV detectors, recent advances in HPLC/mass spectrometry, and a discussion of hardware configurations and interfacing to computers and data acquisition devices. A guide to separations, a glossary of terms, and a programmed self-learning guide are also included.