The Practice of Quantitative Gel Electrophoresis (Advanced Methods in the Biological Sciences)

A practical guide to proven procedures and special methods (including polyacrylamide and agarose electrophoresis, isotachophoresis, and electrofocusing) that are generally applicable to identifying and isolating charged molecules. The book also describes in detail all the equipment required and provides construction plans for those items that are not commercially available. The author has kept theory to a minimum in the interests of readability and usability. The electrophoretic separations he describes are those of native macromolecules as opposed to dissociated and denatured gene products. All the methods have been proved reliable and are used in daily laboratory work. A comprehensive index makes this book an invaluable reference tool for molecular biologists, clinical chemists, pharmaceutical and food chemists, biochemists, and physiologists.