Hematopoietic Stem Cell Protocols (Methods in Molecular Medicine)

A group of leading stem-cell investigators describe in detail the key laboratory methods for investigating hematopoietic stem cells. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and blood cancers such as leukemia.