Bioinformatics Practical Manual

1. ACCESSING BIBLIOGRAPHIC DATABASE FROM NCBI 2. RETRIEVING PROTEIN / NUCLEOTIDE SEQUENCE INFORMATION FROM NCBI DATABASE 3. RESTRICTION MAPPING 4. SIMILARITY SEARCH USING FASTA TOOL FROM EMBL 5. SIMILARITY SEARCH USING BLAST TOOL FROM NCBI 6. PAIRWISE SEQUENCE ALIGNEMENT –EMBOSS SUITE 7. MULTIPLE SEQUENCE ALIGNMENT – CLUSTAL OMEGA 8. EVOLUTIONALRY RELATIONSHIP- PHYLOGENETIC ANALYSIS – TREE VIEW 9. PDB RETRIEVING 10. MOLECULAR VISUALIZATION-RASMOL 11. IDENTIFICATION OF GENE (HMGA2) IN HUMAN GENOME & Primer Designing 12. INTRODUCTION TO AUTODOCK 13. CALCULATION OF SD, VARIANCE, GRAPH PLOT – MS EXCEL 14. OPEN READING FRAME FROM - NCBI 15. RAMACHANDRAN PLOT