This latest volume in the Practical Methods in Electron Microscopy series describes the theory of electron-probe X-ray microanalysis (EPXMA), and the instruments used, and gives practical advice about the preparation of biological specimens and standards for EPXMA. Detailed and fully illustrated descriptions are given of: the theoretical aspects of EPXMA; beam-specimen interactions; the instruments used; preparation of biological specimens; quantification theory; practical aspects of quantification and digital X-ray mapping. The book is essential reading for biologists who are using or which to use the technique of EPXMA.