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Enzymatic chemiluminescent assay of glucose by sequential-injection [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000P6O474
ISBN-13978B000P6O471
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Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
This work reports a sequential-injection analysis (SIA) method for the enzymatic assay of glucose with soluble glucose oxidase (GOD) and on-line sample dilution with chemiluminescence (CL) detection. A zone of sample was aspirated in the holding coil of the SIA manifold and, if necessary, was diluted on-line by means of an auxiliary dilution conduit. Then, a zone of GOD was aspirated adjacent to the sample zone and a stopped-flow period was applied to allow the enzymatic reaction to proceed with production of hydrogen peroxide. Then, zones of a catalyst (Co(II) solution) and alkaline luminol were aspirated into the holding coil. Finally, the flow was reversed and the stacked zones were sent to a flow-cell located in front of a photomultiplier tube (PMT) that monitored the CL intensity. The linear dynamic range was 1x10^-^5-1x10^-^3molL^-^1 glucose, the coefficient of variation at 8x10^-^5molL^-^1 of glucose was s"r=3.1% (n=8), the limit of detection at the 3@s level was c"L=1x10^-^6molL^-^1 and the sampling frequency was 28h^-^1. With on-line dilution by a factor of 1/200, the linear range could be extended up to 0.2molL^-^1 glucose. The advantages of the proposed method are the simple manifold and instrumentation used, the scope for automated on-line dilution, the low consumption of sample and reagents and the elimination of enzyme immobilisation procedures. The method was applied to the analysis of commercial drinks and honey with percent relative errors in glucose determination in the range 100+/-6.1%.
Description:
This work reports a sequential-injection analysis (SIA) method for the enzymatic assay of glucose with soluble glucose oxidase (GOD) and on-line sample dilution with chemiluminescence (CL) detection. A zone of sample was aspirated in the holding coil of the SIA manifold and, if necessary, was diluted on-line by means of an auxiliary dilution conduit. Then, a zone of GOD was aspirated adjacent to the sample zone and a stopped-flow period was applied to allow the enzymatic reaction to proceed with production of hydrogen peroxide. Then, zones of a catalyst (Co(II) solution) and alkaline luminol were aspirated into the holding coil. Finally, the flow was reversed and the stacked zones were sent to a flow-cell located in front of a photomultiplier tube (PMT) that monitored the CL intensity. The linear dynamic range was 1x10^-^5-1x10^-^3molL^-^1 glucose, the coefficient of variation at 8x10^-^5molL^-^1 of glucose was s"r=3.1% (n=8), the limit of detection at the 3@s level was c"L=1x10^-^6molL^-^1 and the sampling frequency was 28h^-^1. With on-line dilution by a factor of 1/200, the linear range could be extended up to 0.2molL^-^1 glucose. The advantages of the proposed method are the simple manifold and instrumentation used, the scope for automated on-line dilution, the low consumption of sample and reagents and the elimination of enzyme immobilisation procedures. The method was applied to the analysis of commercial drinks and honey with percent relative errors in glucose determination in the range 100+/-6.1%.
