![Progestin facilitation of lordosis in rodents involves adenylyl cyclase [An article from: Hormones and Behavior]](https://www.ebooknetworking.net/books/noimage.jpg)
Progestin facilitation of lordosis in rodents involves adenylyl cyclase [An article from: Hormones and Behavior]
Book Details
Author(s)C.A. Frye, A.A. Walf, S.M. Petralia
PublisherElsevier
ISBN / ASINB000P6O4H4
ISBN-13978B000P6O4H2
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Hormones and Behavior, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Increasing cAMP, or activating dopamine type 1 (D"1) or GABA"A/benzodiazepine receptor complexes (GBRs), in the ventral tegmental area (VTA) enhances lordosis of rodents. Whether D"1- and/or GBR-mediated increases in progestin-facilitated lordosis involve the cAMP-synthesizing enzyme, adenylyl cyclase, in the VTA, was investigated. In Experiment 1, ovariectomized estradiol (E"2; 10 @mg at h 0)+progesterone (P; 250 @mg at h 45)-primed hamsters first received bilateral infusions of the adenylyl cyclase inhibitor, 2',5'-dideoxyadenosine (DDA; 12 @mM/side), or vehicle, and then were infused with the D"1 agonist, SKF38393 (100 ng/side), the GBR agonist, muscimol (100 ng/side), or vehicle, to the VTA. Lordosis was evaluated before and 30 min after each infusion. In Experiment 2, ovariectomized, E"2-primed (10 @mg at h 0) rats received VTA infusions of DDA (12 @mM/side) or vehicle; SKF38393 (100 ng/side), muscimol (100 ng/side), or vehicle; and the neurosteroid, 5@a-pregnan-3@a-ol-20-one (3@a,5@a-THP; 100 or 200 ng/side), or @b-cyclodextrin vehicle. Lordosis was assessed before the series of infusions, immediately after drug infusions and 10 or 60 min after 3@a,5@a-THP infusions. Progestin- or progestin plus SKF38393-or muscimol-mediated increases in lordosis were blocked by DDA pretreatment. Thus, in the VTA, progestins' membrane action may involve adenylyl cyclase.
Description:
Increasing cAMP, or activating dopamine type 1 (D"1) or GABA"A/benzodiazepine receptor complexes (GBRs), in the ventral tegmental area (VTA) enhances lordosis of rodents. Whether D"1- and/or GBR-mediated increases in progestin-facilitated lordosis involve the cAMP-synthesizing enzyme, adenylyl cyclase, in the VTA, was investigated. In Experiment 1, ovariectomized estradiol (E"2; 10 @mg at h 0)+progesterone (P; 250 @mg at h 45)-primed hamsters first received bilateral infusions of the adenylyl cyclase inhibitor, 2',5'-dideoxyadenosine (DDA; 12 @mM/side), or vehicle, and then were infused with the D"1 agonist, SKF38393 (100 ng/side), the GBR agonist, muscimol (100 ng/side), or vehicle, to the VTA. Lordosis was evaluated before and 30 min after each infusion. In Experiment 2, ovariectomized, E"2-primed (10 @mg at h 0) rats received VTA infusions of DDA (12 @mM/side) or vehicle; SKF38393 (100 ng/side), muscimol (100 ng/side), or vehicle; and the neurosteroid, 5@a-pregnan-3@a-ol-20-one (3@a,5@a-THP; 100 or 200 ng/side), or @b-cyclodextrin vehicle. Lordosis was assessed before the series of infusions, immediately after drug infusions and 10 or 60 min after 3@a,5@a-THP infusions. Progestin- or progestin plus SKF38393-or muscimol-mediated increases in lordosis were blocked by DDA pretreatment. Thus, in the VTA, progestins' membrane action may involve adenylyl cyclase.
