Identification of an anonymous RFLP DNA probe through multiple [An article from: Bioresource Technology]

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Description:
The genome of Aspergillus niger (MPS-002) was subjected to RAPD fingerprinting using none different random oligonucleotide primers. A 0.7Kb PCR amplicon, generated by primer-3 could be used as a RFLP probe to differentiate A. niger (ATCC 16880) from A. niger (MPS-002). The probe revealed DNA polymorphism internal to two different EcoRI recognition sequences spaced apart at a distance of 0.4Kb within a 4.0Kb EcoRI fragment of the genome of both the strains. Localized genome mapping analysis further revealed that the 0.7Kb RFLP probe was positioned at a distance of 2.7Kb and 0.6Kb from the two ends of a 4.0Kb EcoRI fragment, respectively within the genome of the two strains of A. niger.