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Electrogenerated chemiluminescence biosensor based on Ru(bpy)"3^2^+ and [An article from: Analytica Chimica Acta]
Book Details
Author(s)L. Zhang, Z. Xu, S. Dong
PublisherElsevier
ISBN / ASINB000P6OF8W
ISBN-13978B000P6OF82
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)"3^2^+ and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)"3^2^+ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3x10^-^6M for alcohol (S/N=3) with a linear range from 2.79x10^-^5 to 5.78x10^-^2M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.
Description:
A new electrogenerated chemiluminescence biosensor was fabricated by immobilizing ECL reagent Ru(bpy)"3^2^+ and alcohol dehydrogenase in sol-gel/chitosan/poly(sodium 4-styrene sulfonate) (PSS) organically modified composite material. The component PSS was used to immobilize ECL reagent Ru(bpy)"3^2^+ by ion-exchange, while the addition of chitosan was to prevent the cracking of conventional sol-gel-derived glasses and provide biocompatible microenvironment for alcohol dehydrogenase. Such biosensor combined enzymatic selectivity with the sensitivity of ECL detection for quantification of enzyme substrate and it was much simpler than previous double-layer design. The detection limit was 9.3x10^-^6M for alcohol (S/N=3) with a linear range from 2.79x10^-^5 to 5.78x10^-^2M. With ECL detection, the biosensor exhibited wide linear range, high sensitivity and good stability.
