Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge [An article from: Bioresource Technology]

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Description:
Bacillus coagulans strain IIT-BT S1 isolated from anaerobically digested activated sewage sludge was investigated for its ability to produce H"2 from glucose-based medium under the influence of different environmental parameters. At mid-exponential phase of cell growth, H"2 production initiated and reached maximum production rate in the stationary phase. The maximal H"2 yield (2.28molH"2/molglucose) was recorded at an initial glucose concentration of 2% (w/v), pH 6.5, temperature 37^oC, inoculum volume of 10% (v/v) and inoculum age of 14h. Cell growth rate and rate of hydrogen production decreased when glucose concentration was elevated above 2% w/v, indicating substrate inhibition. The ability of the organism to utilize various carbon sources for H"2 fermentation was also determined.