Enhanced synchronous spectrofluorimetric determination of aflatoxin B"1 in pistachio samples using multivariate analysis [An article from: Analytica Chimica Acta]

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
The determination of aflatoxin B"1 (AFB1) in pistachio has been accomplished by normal and synchronous fluorimetry in combination with some multivariate calibration methods and derivative techniques. Extending the two-dimensional synchronous fluorescence scan to a three-dimensional total synchronous fluorescence scan was used to obtain the optimized @D@l for AFB1 in pistachio sample. The methods are based on the enhanced fluorescence of AFB1 by @b-cyclodextrine in 10% (v/v) methanol-water solution. Twenty-six pistachio samples containing AFB1 in the range 0-15ppb were used as calibration set. Eighteen combinational methods were tested to make best model for prediction of AFB1 and finally best results obtained using a method based on synchronous fluorimetry in combination with multiple linear regressions (MLR). For concentrations ranging from 0 to 15ppb of AFB1 in 22 pistachio samples as prediction set, the values of root mean square difference (RMSD) and relative error of prediction (REP) using MLR were 0.328 and 4.453%, respectively were observed. Two naturally contaminated pistachio samples were analyzed by synchronous fluorimetry using MLR and compared with HPLC results.