Immobilization of cholesterol esterase and cholesterol oxidase onto sol-gel films for application to cholesterol biosensor [An article from: Analytica Chimica Acta]

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Description:
Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol-gel films. The tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV-vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol-gel/ChEt/ChOx reveal thermal stability up to 55^oC, response time as 180s, linearity up to 780mgdL^-^1 (12mM), shelf life of 1 month, detection limit of 12mgdL^-^1 and sensitivity as 5.4x10^-^5Abs.mg^-^1dL^-^1.