The ultimate veal calf reference experiment: Hormone residue analysis data obtained by gas and liquid chromatography tandem mass spectrometry [An article from: Analytica Chimica Acta]

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
A lifetime controlled reference experiment has been performed using 42 veal calves, 21 males and 21 females which were fed and housed according to European regulations and common veterinary practice. During the experiment feed, water, urine and hair were sampled and feed intake and growth were monitored. Thus for the first time residue analysis data were obtained from guaranteed lifetime-untreated animals. The analysis was focused on the natural hormones estradiol and testosterone and their metabolites, on 17@b- and 17@a-nortestosterone, on 17@b- and 17@a-boldenone and androsta-1,4-diene-3,17-dione (ADD), and carried out by gas chromatography tandem mass spectrometry (GC/MS/MS), an estrogen bioassay and liquid chromatography (LC) MS/MS. Feed, water and hair samples were negative for the residues tested. Female calf urines showed occasionally low levels of 17@a-estradiol and 17@a-testosterone. On one particular sampling day male veal calf urines showed very high levels of 17@a-testosterone (up to 1000ngmL^-^1), accompanied by lower levels of estrone and 17@b-testosterone. Despite these extreme levels of natural testosterone, 17@b-boldenone was never detected in the same urine samples; even 17@a-boldenone and ADD were only occasionally beyond CC@a (maximum levels 2.7ngmL^-^1). The data from this unique experiment provide a set of reference values for steroid hormones in calf urine and demonstrate that 17@b-boldenone is not a naturally occurring compound in urine samples.