Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1 [An article from: Bioresource Technology]

This digital document is a journal article from Bioresource Technology, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
A newly isolated strain of Kluyveromyces marxianus YS-1 was used for the production of extra cellular inulinase in a medium containing inulin, meat extract, CaCl"2 and sodium dodecyl sulphate (SDS). Fermentation medium pH 6.5, cultivation temperature 30^oC and 5% (v/v) inoculum of 12h-old culture were optimal for enzyme production (30.8IU/ml) with a fermentation time of 72h at shake flask level. Raw inulin (2%, w/v) extracted from dahlia tubers by processing at 15kg/cm^2 for 10min was optimum for bioreactor studies. Maximum enzyme production (55.4IU/ml) was obtained at an agitation rate of 200rpm and aeration of 0.75vvm in a stirred tank reactor with a fermentation time of 60h.