![Community composition and activities of denitrifying bacteria from adjacent agricultural soil, riparian soil, and creek sediment in Oregon, USA [An article from: Soil Biology and Biochemistry]](https://www.ebooknetworking.net/books/B00/0RQ/bigB000RQZPTI.jpg)
Community composition and activities of denitrifying bacteria from adjacent agricultural soil, riparian soil, and creek sediment in Oregon, USA [An article from: Soil Biology and Biochemistry]
Book Details
Author(s)J.J. Rich, D.D. Myrold
PublisherElsevier
ISBN / ASINB000RQZPTI
ISBN-13978B000RQZPT2
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Soil Biology and Biochemistry, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
We examined denitrifying bacteria from wet soils and creek sediment in an agroecosystem in Oregon, USA that received inputs of nitrogen (N) fertilizer. Our objective was to determine the variation in denitrifying community composition and activities across three adjacent habitats: a fertilized agricultural field planted to perennial ryegrass, a naturally vegetated riparian area, and creek sediment. Using C"2H"2 inhibition, denitrifying enzyme and N"2O-reductase activities were determined in short-term incubations of anaerobic slurries. A key gene in the denitrification pathway, N"2O reductase (nosZ), served as a marker for denitrifiers. Mean denitrifying enzyme activity (DEA) was similar among habitats, ranging from 0.5 to 1.8@mgNg^-^1 dry soilh^-^1. However, the ratio of N"2O production, without C"2H"2, to DEA was substantially higher in riparian soil (0.64+/-0.02; mean+/-standard error, n=12) than in agricultural soil (0.19+/-0.02) or creek sediment (0.32+/-0.03). Mean N"2O-reductase activity ranged from 0.5 to 3.2@mgNg^-^1 dry soilh^-^1, with greater activity in agricultural soil than in riparian soil. Denitrifying community composition differed significantly among habitats based on nosZ terminal-restriction fragment length polymorphisms. The creek sediment community was unique. Communities in the agricultural and riparian soil were more closely related but distinct. A number of unique nosZ genotypes were detected in creek sediment. Sequences of nosZ obtained from riparian soil were closely related to nosZ from Bradyrhizobium japonicum. Although nosZ distribution and N"2O-reductase activity differed among habitats, relationships between activity and community composition appeared uncoupled across the agroecosystem.
Description:
We examined denitrifying bacteria from wet soils and creek sediment in an agroecosystem in Oregon, USA that received inputs of nitrogen (N) fertilizer. Our objective was to determine the variation in denitrifying community composition and activities across three adjacent habitats: a fertilized agricultural field planted to perennial ryegrass, a naturally vegetated riparian area, and creek sediment. Using C"2H"2 inhibition, denitrifying enzyme and N"2O-reductase activities were determined in short-term incubations of anaerobic slurries. A key gene in the denitrification pathway, N"2O reductase (nosZ), served as a marker for denitrifiers. Mean denitrifying enzyme activity (DEA) was similar among habitats, ranging from 0.5 to 1.8@mgNg^-^1 dry soilh^-^1. However, the ratio of N"2O production, without C"2H"2, to DEA was substantially higher in riparian soil (0.64+/-0.02; mean+/-standard error, n=12) than in agricultural soil (0.19+/-0.02) or creek sediment (0.32+/-0.03). Mean N"2O-reductase activity ranged from 0.5 to 3.2@mgNg^-^1 dry soilh^-^1, with greater activity in agricultural soil than in riparian soil. Denitrifying community composition differed significantly among habitats based on nosZ terminal-restriction fragment length polymorphisms. The creek sediment community was unique. Communities in the agricultural and riparian soil were more closely related but distinct. A number of unique nosZ genotypes were detected in creek sediment. Sequences of nosZ obtained from riparian soil were closely related to nosZ from Bradyrhizobium japonicum. Although nosZ distribution and N"2O-reductase activity differed among habitats, relationships between activity and community composition appeared uncoupled across the agroecosystem.
