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Pressurized liquid extraction of isoflavones from soybeans [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000RR04HU
ISBN-13978B000RR04H8
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Isoflavone derivatives from freeze-dried soybeans were extracted by pressurized liquid extraction (PLE) and determined by reverse-phase high performance liquid chromatography (HPLC) with both photo diode array and mass spectrometry (MS) detection. Both real and spiked samples were used in the development of the method. Several extraction solvents (methanol (MeOH) and ethanol (EtOH), 30-80% in water and water), temperatures (60-200^oC), pressures (100-200atm), as well as the sample size (0.5-0.05g) and cycle length (5-10min) were studied for the optimization of the extraction protocol. The optimized extraction conditions for quantitative recoveries were: 0.1g of sample, 100^oC, three (7min) static extraction cycles and ethanol 70% as extracting solvent. The stability of the isoflavones during the PLE was also determined. Under PLE conditions, degradation of malonyl glucoside forms of the isoflavones takes place using temperatures higher than 100^oC whereas degradation of glucosides takes place above 150^oC. Using the optimized protocol, isoflavones can be extracted from freeze-dried soybeans without degradation.
Description:
Isoflavone derivatives from freeze-dried soybeans were extracted by pressurized liquid extraction (PLE) and determined by reverse-phase high performance liquid chromatography (HPLC) with both photo diode array and mass spectrometry (MS) detection. Both real and spiked samples were used in the development of the method. Several extraction solvents (methanol (MeOH) and ethanol (EtOH), 30-80% in water and water), temperatures (60-200^oC), pressures (100-200atm), as well as the sample size (0.5-0.05g) and cycle length (5-10min) were studied for the optimization of the extraction protocol. The optimized extraction conditions for quantitative recoveries were: 0.1g of sample, 100^oC, three (7min) static extraction cycles and ethanol 70% as extracting solvent. The stability of the isoflavones during the PLE was also determined. Under PLE conditions, degradation of malonyl glucoside forms of the isoflavones takes place using temperatures higher than 100^oC whereas degradation of glucosides takes place above 150^oC. Using the optimized protocol, isoflavones can be extracted from freeze-dried soybeans without degradation.
