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Multi sulfonamide screening in porcine muscle using a surface plasmon resonance biosensor [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000RR3G8Y
ISBN-13978B000RR3G89
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A binding protein displaying broad-spectrum cross-reactivity within the sulfonamide group was used in conjunction with a sulfonamide specific sensor chip and a surface plasmon resonance biosensor to develop a rapid broad spectrum screening assay for sulfonamides in porcine muscle. Results for 40 samples were available in just over 5h after the completion of a simple sample preparation protocol. Twenty sulfonamide compounds were detected. Acetylated metabolites were not recognised by the binding protein. Limit of detection (mean-three times standard deviation value when n=20) was calculated to be 16.9ngg^-^1 in tissue samples. Intra-assay precision (n=10) was calculated at 4.3 %CV for a sample spiked at 50ngg^-^1 with sulfamethazine, 3.6 %CV for a sample spiked at 100ngg^-^1 with sulfamethazine, 7.2 %CV for a sample spiked at 50ngg^-^1 with sulfadiazine and 3.1 %CV for a sample spiked at 100ngg^-^1 with sulfadiazine. Inter-assay precision (n=3) was calculated at 9.7 %CV for a sample spiked at 50ngg^-^1 with sulfamethazine, 3.8 %CV for a sample spiked at 100ngg^-^1 with sulfamethazine, 3.5 %CV for a sample spiked at 50ngg^-^1 with sulfadiazine and 2.8 %CV for a sample spiked at 100ngg^-^1 with sulfadiazine.
Description:
A binding protein displaying broad-spectrum cross-reactivity within the sulfonamide group was used in conjunction with a sulfonamide specific sensor chip and a surface plasmon resonance biosensor to develop a rapid broad spectrum screening assay for sulfonamides in porcine muscle. Results for 40 samples were available in just over 5h after the completion of a simple sample preparation protocol. Twenty sulfonamide compounds were detected. Acetylated metabolites were not recognised by the binding protein. Limit of detection (mean-three times standard deviation value when n=20) was calculated to be 16.9ngg^-^1 in tissue samples. Intra-assay precision (n=10) was calculated at 4.3 %CV for a sample spiked at 50ngg^-^1 with sulfamethazine, 3.6 %CV for a sample spiked at 100ngg^-^1 with sulfamethazine, 7.2 %CV for a sample spiked at 50ngg^-^1 with sulfadiazine and 3.1 %CV for a sample spiked at 100ngg^-^1 with sulfadiazine. Inter-assay precision (n=3) was calculated at 9.7 %CV for a sample spiked at 50ngg^-^1 with sulfamethazine, 3.8 %CV for a sample spiked at 100ngg^-^1 with sulfamethazine, 3.5 %CV for a sample spiked at 50ngg^-^1 with sulfadiazine and 2.8 %CV for a sample spiked at 100ngg^-^1 with sulfadiazine.
