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Detection of chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, prawn and milk using a surface plasmon resonance ... [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000RR3G9S
ISBN-13978B000RR3G96
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MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Immunochemical screening assays using surface plasmon resonance have been developed for chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, prawn and cows' milk using a sensor chip coated with a chloramphenicol derivative and an antibody. The antibody cross-reacted with chloramphenicol glucuronide 73.8% (poultry), 69.2% (honey), 75.7% (prawn) and 84.8% (milk). There was no cross-reaction with similar drugs or other commonly used antibiotics. The assay allowed the direct analysis of bovine milk (fat content ~3.5%). Poultry, honey and prawn samples were extracted with ethyl acetate followed by analysis on the biosensor. The decision limits (CC@a) for each assay were determined as: poultry (0.005@mgkg^-^1), honey (0.02@mgkg^-^1), prawn (0.04@mgkg^-^1) and milk (0.04@mgkg^-^1) and the detection capabilities (CC@b) were 0.02, 0.02, 0.07 and 0.05@mgkg^-^1, respectively. Poultry muscle, honey and milk were spiked at 0.1@mgkg^-^1 and prawn at 0.15@mgkg^-^1 and the intra-assay precision (n=10) calculated as 10.5, 5.0, 4.6 and 8.8%, respectively. Between run precision (n=3) performed at the same levels yielded the following results: 3.0% (poultry), 4.7% (honey), 7.6% (milk) and 5.5% (prawn).
Description:
Immunochemical screening assays using surface plasmon resonance have been developed for chloramphenicol and chloramphenicol glucuronide residues in poultry muscle, honey, prawn and cows' milk using a sensor chip coated with a chloramphenicol derivative and an antibody. The antibody cross-reacted with chloramphenicol glucuronide 73.8% (poultry), 69.2% (honey), 75.7% (prawn) and 84.8% (milk). There was no cross-reaction with similar drugs or other commonly used antibiotics. The assay allowed the direct analysis of bovine milk (fat content ~3.5%). Poultry, honey and prawn samples were extracted with ethyl acetate followed by analysis on the biosensor. The decision limits (CC@a) for each assay were determined as: poultry (0.005@mgkg^-^1), honey (0.02@mgkg^-^1), prawn (0.04@mgkg^-^1) and milk (0.04@mgkg^-^1) and the detection capabilities (CC@b) were 0.02, 0.02, 0.07 and 0.05@mgkg^-^1, respectively. Poultry muscle, honey and milk were spiked at 0.1@mgkg^-^1 and prawn at 0.15@mgkg^-^1 and the intra-assay precision (n=10) calculated as 10.5, 5.0, 4.6 and 8.8%, respectively. Between run precision (n=3) performed at the same levels yielded the following results: 3.0% (poultry), 4.7% (honey), 7.6% (milk) and 5.5% (prawn).
