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Determination of thiazinamium, promazine and promethazine in pharmaceutical formulations using a CZE method [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000RR3ILY
ISBN-13978B000RR3IL3
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Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
We present the validation of a developed method using capillary zone electrophoresis (CZE) for quantitative analysis of three phenothiazines: thiazinamium methylsulphate (TMS), promazine hydrochloride (PMH) and promethazine hydrochloride (PTH) in pharmaceutical formulations. The influence of various parameters affecting the CZE separation (buffer pH and concentration, acetonitrile percentage, capillary temperature and applied voltage) was investigated by means of multivariate optimization using experimental designs so as to obtain the highest efficiency. The method allows the separation of the phenothiazines in 5.0min at optimized conditions: 100mM tris(hydroxymethyl)-aminomethane (Tris) buffer at a pH 8.0, 15% acetonitrile, capillary with 58.5cm in length at 25^oC and a voltage of 30kV. Detection occurred at 254nm. Acceptable precision (relative standard deviation (R.S.D.) 5.3%) and linearity were achieved using the internal standard (IS) method. The limits of detection (LODs) were 2.8@mgml^-^1 for TMS and 3.3@mgml^-^1 for PMH and PTH. The method has been successfully applied in the analysis of pharmaceutical formulations.
Description:
We present the validation of a developed method using capillary zone electrophoresis (CZE) for quantitative analysis of three phenothiazines: thiazinamium methylsulphate (TMS), promazine hydrochloride (PMH) and promethazine hydrochloride (PTH) in pharmaceutical formulations. The influence of various parameters affecting the CZE separation (buffer pH and concentration, acetonitrile percentage, capillary temperature and applied voltage) was investigated by means of multivariate optimization using experimental designs so as to obtain the highest efficiency. The method allows the separation of the phenothiazines in 5.0min at optimized conditions: 100mM tris(hydroxymethyl)-aminomethane (Tris) buffer at a pH 8.0, 15% acetonitrile, capillary with 58.5cm in length at 25^oC and a voltage of 30kV. Detection occurred at 254nm. Acceptable precision (relative standard deviation (R.S.D.) 5.3%) and linearity were achieved using the internal standard (IS) method. The limits of detection (LODs) were 2.8@mgml^-^1 for TMS and 3.3@mgml^-^1 for PMH and PTH. The method has been successfully applied in the analysis of pharmaceutical formulations.
