Determining morphine in biologic fluids of rats by gas chromatography-mass spectrometry [An article from: Analytica Chimica Acta]

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Description:
This study describes a sensitivity and selectivity approach based on gas chromatography-mass spectrometry to determinate morphine levels in rat biologic fluids. Optimized experimental conditions of solid-phase extraction (SPE), including pH of the sample, solvent composition, and various sorbents, are examined herein. The largest recovery is greater than 94.0% for morphine, is achieved with a mixed sorbent C"8/SCX and mixed elution CHCl"3/iso-propanol (9:1) at pH 6.8. Acetylation accomplished with acetic anhydride is utilized to derivatize morphine to 3,6-diacetylmorphine for GC/MS analysis. The proposed method was precise and a wide linear range from 0.5 to 500ngmL^-^1. A detection limit of 0.31ngmL^-^1 in urine is achieved. The maximum ratio of morphine glucuronide to morphine in the blood of rat is 6 to 1. A terminal half-life of morphine in blood is calculated as around 120min.