This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.
Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.
- Includes a glossary of over 200 terms important for understanding molecular biology
- Uses an inexpensive source of eukaryotic cells - great for schools on a budget
- Includes Methods Locator that provides instant access to the latest methods
- Contain clearly written, easy-to-follow, student-tested instructions:
- Sterile techniques
- Phage titration
- Gel electrophoresis of DNA
- Restriction enzyme digestion
- Plasmid isolation
- Transformation of E. Coli
- Recombinant DNA cloning
- Nick translation labeling
- Nonradioactive primer labelling
- Nonradioactive DNA detection
- Southern blotting
- Colony hybridization
- Purification of plant DNA
- RNA purification
- Northern blotting
- Purification of poly A+ RNA
- Polymerase chain reaction (PCR)