Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.
- Contains a comprehensive collection of transgenic animal and gene transfer methods
- Discusses background and introduction to techniques and animal systems
- Teaches practical step-by-step protocols
- Fully revised with updates to reflect state-of-the-art technology and associated changes to date