A Practical Guide to Enzymology (Biochemistry: A Series of Monographs)

The only text providing practical guidelines to a wide range of topics important to the practicing enzymologist. It offers advice on how solvents, salts, substrates,and denaturants affect enzyme function and stability; developing a scheme for purifying an enzyme from different kinds of tissue; applying different techniques for fractionating proteins; characterizing proteins by determining molecular weight, isoelectric pH, amino acid composition and sequence, ligand binding, and enzyme kinetics. Basic guidelines to procedures and hints for the operation of a technique are also included.