Methods in Molecular Biology, Vol. 8: Practical Molecular Virology- Viral Vectors for Gene Expression

Mary K. L. Collins has assembled in Practical Molecular Virology a vanguard collection of readily repeatable methods for gene transfer and expression using a variety of recombinant viral vectors.

In keeping with the established tradition of the series, each technique is presented in an easy-to-follow format designed to work for the novice as well as the seasoned expert. Chapters cover: • life cycles of specific retroviruses and how recombinant vectors are constructed • PCR techniques • poliovirus vectors • herpesvirus vectors • syncytial assays • cell lineage studies • baculovirus and adenovirus vectors • SV40 and EBV vectors • viruses in gene transfer to eukaryotic cells

The wealth of material devoted to recombinant retroviral methods and their applications make Practical Molecular Virology and extremely timely volume, one that will find widespread use throughout biological and biomedical research.