Cell Migration in Inflammation and Immunity: Methods and Protocols (Methods in Molecular Biology)

Leading cell biologists and immunologists present their most useful and innovative techniques for studying the molecular and cellular basis of cell migration in the inflammatory process. The authors provide a series of focused, cutting-edge techniques proceeding from the in vitro analysis of cell migration and the molecular mechanisms underlying this process, to methodologies for the analysis of cell migration in vivo. Experimental approaches useful in establishing the role of cell migration in the pathogenesis of both acute and chronic inflammatory diseases are emphasized. Methods for the analysis of rapid leukocyte adhesion under flow conditions in vitro are described, which may prove especially fruitful for scientists exploring the molecular mechanisms underlying vascular recognition and leukocyte-endothelium interaction. Each fully tested protocol includes an introduction explaining the principle behind the technique, equipment and reagent lists, and tips on troubleshooting and how to avoid known pitfalls.