@a-Galactosidase production by Aspergillus oryzae in solid-state fermentation [An article from: Bioresource Technology]

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Description:
Comparisons were made for @a-galactosidase production using red gram plant waste (RGPW) with wheat bran (WB) and other locally available substrates using the fungus Aspergillus oryzae under solid-state fermentation (SSF). RGPW proved to be potential substrate for @a-galactosidase production as it gave higher enzyme titers (3.4U/g) compared to WB (2.7U/g) and other substrates tested. Mixing WB with RGPW (1:1, w/w) resulted enhanced @a-galactosidase yield. The volume of moistening agent in the ratio of 1:2 (w/v), pH 5.5 and 1ml (1x10^6 spores) of inoculum volume and four days incubation were optimum for @a-galactosidase production. Increase in substrate concentration (RGPW+WB) did not decrease enzyme yield in trays.