Analysis for zearalenone and @a-zearalenol in cereals and swine feed using accelerated solvent extraction and liquid chromatography with fluorescence ... [An article from: Analytica Chimica Acta]

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Description:
A fast and accurate method for the determination of zearalenone (ZON) and @a-zearalenol (@a-ZOL) in ground wheat samples has been optimised using accelerated solvent extraction (ASE) and liquid chromatography (LC) with fluorescence detection. The effects of the experimentally controllable ASE parameters (solvent composition, temperature, pressure, number of extraction cycles and sample cell size) on the extraction efficiencies of the target analytes have been investigated using wheat samples fortified at the ngg^-^1 level. The optimum extraction conditions were obtained using a 11mL ASE cell, methanol/acetonitrile (50:50v/v) as the extraction solvent, 1500psi, 50^oC, a 5min static time and a 60% flush volume. The extracted samples were analysed using LC with fluorescence detection (@l"e"x"c=271 and @l"e"m=452nm). The stationary phase was a polar endcapped C"1"8 column and acetonitrile/methanol/water 10/55/35 (v/v/v, 15mM ammonium acetate) at a flow rate of 1.0mLmin^-^1 was used as the mobile phase. The injection volume was set at 8@mL. Mean recovery rates of 98.4% for @a-ZOL (RSD