Direct sub-ppt detection of the endocrine disruptor ethinylestradiol in water with a chemiluminescence enzyme-linked immunosorbent assay [An article from: Analytica Chimica Acta]

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Description:
A chemiluminescence ELISA for the direct detection of ethinylestradiol (EE2) in water at sub-ppt levels was developed and validated. At a signal-to-noise ratio of three the detection limit is 0.2+/-0.1ngL^-^1, at a ratio of 10 the LOQ is found to be 1.4+/-0.8ngL^-^1. Based on a conservatively calculated precision profile the analytical working range is established from 0.8 to 100ngL^-^1. The ELISA was tested in four different matrices, including surface water and effluent of sewage treatment plants. All measurements were validated using an LC-MS/MS method. Typical results were consistent in both methods below 1ngL^-^1. Using this chemiluminescence ELISA facilitates for the first time the direct detection of EE2 at ecotoxicologically relevant concentrations.