Study on the enhancement of electrochemiluminescence of luminol-H"2O"2 system by sulphonated cobalt(II) phthalocyanine [An article from: Analytica Chimica Acta]

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Description:
The main aim of this paper is to explore a new label for electrochemiluminescence (ECL) bioassays. Tetra-substituted sulphonated cobalt(II) (CoTSPc) acting as a mimetic peroxidase of horseradish peroxidase was first found to be able to catalyze the ECL reaction of luminol-H"2O"2 at a glass carbon electrode. CoTSPc also retained its ECL catalytic activity when it was conjugated to BSA. Moreover, the enhanced ECL intensity of the luminol-H"2O"2 system at pH 7.4 was proportional to the amount of target bovine serum albumin (BSA). The spectrum characteristics of CoTSPc-BSA conjugate was examined. The effects of the pH, reaction medium of the solution, the concentration of luminal, H"2O"2 and the electrochemical scanning mode were also investigated. Although refinements in the technique are still necessary before it can be used in practice, a new ECL probe for protein, CoTSPc, has been preliminarily developed.