Determination of tobacco alkaloids by gas chromatography-mass spectrometry using cloud point extraction as a preconcentration step [An article from: Analytica Chimica Acta]

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Description:
The feasibility of employing cloud point extraction (CPE) as a simple and effective alternative for recovery of alkaloids from tobacco samples followed by GC-MS analysis is demonstrated. An aqueous surfactant solution containing 5% Triton X114 was used for extraction of tobacco alkaloids. Then, the analytes were back-extracted with ultrasonic assistance from the surfactant-rich phase into dichloromethane. No other cleanup step preceded GC-MS analysis. Seven alkaloids in tobacco sample with different concentrations were analyzed simultaneously. Results show that the recovery of nicotine is 80.4% and the limit of detection (LOD) is 7.1@mgg^-^1. The relative standard deviations for the seven alkaloids are in the range of 2.77-9.97%. The results yielded by the proposed method were almost identical with those achieved by the more laborious industrial standard method, i.e., the continuous flow method.