Isolation and characterization of indigenous copper-resistant actinomycete strains [An article from: Chemie der Erde - Geochemistry - Interdisciplinary Journal for Chemical]

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Description:
Fifty actinomycetes were isolated from copper contaminated and non-contaminated area. Primary qualitative screening assays showed that 100% of the isolated microorganisms of the contaminated area were resistant up to 80mgL^-^1 of CuSO"4. On the other hand, 100% of isolates from non-contaminated area grew at 16mgL^-^1, 87.4% at 40mgL^-^1 and only 19.4% of them were capable of growing at 80mgL^-^1 of CuSO"4. The semiquantitative assay showed that the isolated strains from the sediments of the contaminated site were resistant up to the highest concentration tested (1000mgL^-^1) with the exception of AB2C strain; however, the strains isolated from non-contaminated sediments were sensitive to Cu^2^+ concentrations higher than 200 and 400mgL^-^1, respectively. Microbial growth of AB0 strain in presence of 39mgL^-^1 copper showed an inhibition of 32% after 6 days of incubation as compared to the control, and copper residual concentration indicated a reduction in the supernatant of 71.2% after 6 days of incubation: pellet acid digestion proved that copper was accumulated by the cells. 16S rDNA restriction digestion of 1300bp amplicons with CfoI and HpaII showed only one restriction pattern for all the strains and it matched with the control, Streptomyces coelicolor.