Genetic diversity of a natural population of Rhizobium leguminosarum biovar trifolii analysed from field nodules and by a plant infection technique [An article from: Soil Biology and Biochemistry]

This digital document is a journal article from Soil Biology and Biochemistry, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
We compared trapping of Rhizobiumleguminosarum biovar trifolii isolates under field soil and non-soil environmental conditions. Isolates were obtained from white clover (Trifoliumrepens L.) and red clover (T. pratense L.) grown directly in the field and from plants inoculated with soil from the same site using a plant infection technique. Isolates were identified by genomic polymerase chain reaction (PCR) fingerprinting using primers derived from the enterobacterial repetitive intergenic consensus (ERIC) sequences. The isolates trapped from soil dilutions in the laboratory included a number of major ERIC types that were not found in the field trapped nodules, suggesting that sampling of clover Rhizobium strains from soil dilutions may not be representative of the field nodulating population.