Antimicrobial activity of delaminated aminopropyl functionalized magnesium phyllosilicates: Scienceworldlib.com

In this study, dispersed aminopropyl functionalized magnesium phyllosilicates (AMP clay) were tested for
antimicrobial activity against various Gram negative and Gram positive bacteria including multidrug resistant
bacterial strains and fungi. The AMP clay strongly inhibits the growth of microorganisms such asEscherichia
coli, Staphylococcus aureusandCandida albicans. The inhibition of microbial growth by AMP clay is attributed
to the amino propyl groups and their charge interactions. The bactericidal effect of AMP clay occurred within
1 h, and 95% killing was observed within 2 h. In addition, AMP clay could kill microbes by disrupting
membrane integrity and thus essential components inside the cells. This effect was also evaluated through
membrane depolarization assays and measured by the release of intracellular enzymes. The assessment of cell
damage by the AMP clay was obtained by scanning electron microscopy (SEM) observation. Moreover, AMP
clay activity was stably maintained after a long storage time of up to 30 days at different temperature
conditions. The goals of this study were to synthesize the dispersed AMP clay from a commercially available
substrate, which can be applied to various biomedical applications and environmental protection