Molecular Biology: A Project Approach

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.

Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

• Includes a glossary of over 200 terms important for understanding molecular biology
• Uses an inexpensive source of eukaryotic cells - great for schools on a budget
• Includes Methods Locator that provides instant access to the latest methods
• Contain clearly written, easy-to-follow, student-tested instructions:
• Sterile techniques
• Phage titration
• Gel electrophoresis of DNA
• Restriction enzyme digestion
• Plasmid isolation
• Transformation of E. Coli
• Recombinant DNA cloning
• Nick translation labeling
• Nonradioactive primer labelling
• Nonradioactive DNA detection
• Southern blotting
• Colony hybridization
• Purification of plant DNA
• RNA purification
• Northern blotting
• Purification of poly A+ RNA
• Polymerase chain reaction (PCR)