Enzyme Histochemistry: A Laboratory Manual of Current Methods (Royal Microscopical Society Microscopy Handbooks)

The functional analysis of macromolecular structures in tissues and cells has been greatly enhanced by advances in histochemistry and cytochemistry. Enzyme histochemistry is becoming particularly important as new methods succeed in demonstrating and quantifying the activity of key regulatory enzymes. The specificity, precision, and reproducibility of enzyme histochemical methods are constantly improving. This practical laboratory handbook contains a selection of the most important enzyme histochemical techniques currently available for light microscopy. The methods included here were chosen because of their reliability and specificity, and all are clearly detailed in easy-to-follow protocols. The introductory chapter provides a good theoretical background to enzyme histochemistry, and the book will be of interest to all researchers in cell biology, pathology, biochemistry, and cell physiology.