Essential Molecular Biology: A Practical Approach 2-vol. set (The Practical Approach Series)

The two volumes of Essential Molecular Biology: A Practical Approach provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume I concentrates on the preparation, purification and handling of nucleic acids, and includes chapters on microbiological procedures and the construction and use of recombinant DNA molecules. Volume II covers the procedures needed for identification of specific genes and the study of their structure and cellular expression. Topics covered include preparation of gene libraries, hybridization methods, nucleic acid sequencing, DNA mapping, and DNA amplification by the polymerase chain reaction. Each chapter is written in an accessible style by an acknowledged expert in that field with the emphasis on providing sound technical instruction, in combination with the necessary theoretical background and useful troubleshooting guidance. This book brings powerful techniques to the forefront of molecular biology research. Together, the two volumes provide a comprehensive molecular biology manual tailored specially for the novice.