Gene Probes 2: A Practical Approach (Practical Approach Series)

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. The advent of solid supports, new D.N.A. labeling techniques, and P.C.R. technology has created a need for an up-to-date practical manual in this area. Gene Probes (Applications), describes techniques for specific research goals. It covers: hybridization strategies; choosing probes; screening and analysis of D.N.A. libraries, including the use of P.C.R.; blotting techniques, gene targeting; and analysis of human genetic mutations. Its companion volume, Gene Probes 1, describes the wide rage of techniques now available for the purification, synthesis, labeling, detection, and quantitation of probes.