Laboratory Protocols for Conditional Gene Targeting

Conditional gene targeting is an emerging technique for introducing mutations into the genes of laboratory mice. This collection of protocols comes from one of the world's premier laboratories for gene targeting--the Institute of Genetics in Cologne--and is written for newcomers and more experienced researchers in the field. Topics include the Cre/loxP recombination system and gene targeting in ES cells; gene targeting vectors; chromosomal translocations; and identification of homologous recombinants.