Molecular Biological Methods for Bacillus (Modern Microbiological Methods)

This series aims to identify specialist areas in microbiology and provide up-to-date methodological handbooks to aid microbiologists at the laboratory bench. The books are directed primarily toward active research workers but are structured so as to serve as an introduction within a speciality for graduate students and scientists entering microbiology from related disciplines. This book focuses on the growth, maintenance and techniques associated with Bacillus. It deals with genetic analysis and then discusses the use of plasmids, transposons and integrational vectors and gene cloning techniques for Bacillus. The book goes on to consider the measurement of gene expression in Bacillus and the mechanisms of DNA repair and replication in this organism. The Bacillus cell envelope and secretion are examined and sporulation is discussed. Finally the book discusses the culturing and maintenance of bacteriophages and provides physical and genetic maps of Bacillus subtilis. Methods and references are given at the end of each section.