Introduction to Confocal Fluorescence Microscopy, Second Edition (SPIE Tutorial Texts in Optical Engineering Vol. TT69)

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Contents

- Symbols and abbreviations
- Preface
- Confocal fluorescence microscopy
- Implementation
- Practical limits
- Digitization
- Miscellaneous topics
- Appendix A: Elements of optical theory
- Appendix B: Formulas, relations and definitions
- Appendix C: Fourier theory
- Bibliography
- Index