Flavoprotein Protocols (Methods in Molecular Biology)

This collection of the most important methodologies for the analysis of the structure and function of flavoproteins ranges from wet to dry chemistry. These step-by-step methods-developed by leading experimentalists to work reliably-include the most commonly used techniques as well as a wide range of spectrophotometric protocols that allow direct monitoring of flavins in real time, in the steady state, or as reaction intermediates. Among the techniques detailed are freeze-quench methods, EPR spectroscopy, CD spectroscopy, vibrational spectroscopy (SERRS), and nuclear magnetic resonance. Also discussed are a variety of therapeutic applications for flavins and flavoproteins that go far beyond the use of riboflavin as a vitamin supplement. Flavoprotein Protocols offers to both experts in the field and to those working on a flavin-containing protein for the first time a wealth of proven methods and laboratory experience that will enhance their understanding of structure/function relationships in the proteins and of the effects of the protein environment on flavin chemistry.