Antigen Processing and Presentation Protocols (Methods in Molecular Biology)

Well-recognized and innovative experimentalists detail their cutting-edge methods for studying the antigen processing and presentation. Drawing on expertise from biochemistry, cell biology, and immunology, they describe step-by-step methods designed to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to the MHC/peptide complex. Emphasis is given those technical steps critical for experimental success that are often omitted from methods published in the primary literature. Eminently accessible and state-of-the-art, Antigen Processing and Presentation Protocols provides both new and experienced investigators with highly practical tools that will extend the questions that can be asked, and effectively be answered, concerning antigen processing/presentation.