Essentials of Enzymology

Essentials of enzymology provides a concise information on the most important areas enzymology that may serve as course material for an advanced course in enzymology designed for undergraduate science degree programme, especially, BSc (Hons) biochemistry final year. In the first part, the nature of enzymes, location, and distribution in cell compartments as well as methods of isolation acid purification procedures are well documented. Then the basic structures of enzymology, organization, and analytical techniques for their quantization are described in comprehensive manner. Subsequently, the kinetics of the catalytic action in diverse experimental conditions are presented in a simple, not-too-complex mathematical approach. Thereafter, the mechanisms of catalysis acid regulation, of enzyme activity are discussed precisely, so also is the active site of enzymes and how this could be identified unambiguously using specific labels. Lastly, the specific applications of enzymes in diagnostic medicine, industry, and the new emerging area of enzyme biotechnology and bioinformatics are presented.