Genetic Recombination: Reviews and Protocols (Methods in Molecular Biology)

Genetic recombination is any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. This book presents leading peer-reviewed protocols to carry out recombinant investigations in the lab. Part I provides approaches and model systems for studying several aspects of recombination in a variety of eukaryotic organisms and in mammalian parasites. Part II describes approaches for using recombination as a reporter of genomic instability in lower and higher eukaryotes. Part III discusses various methods and approaches for targeted genomic manipulation in higher and lower eukaryotes, while the final part presents biochemical analyses useful for furthering the understanding of recombination mechanisms, thus making this an invaluable book for both the novice and the established researcher in the field of recombination.