Egg Yolk Immunoglobulins(IgY)and Ovotransferrin of Chicken Egg: Simple Purificaiton of IgY and Ovotransferrin and Antimicrobial Activity of ... Escherichia coli O157:H7 and L. monocytogenes

The protocol developed for IgY purification indicated approximately 74% recovery rate and 80% purity, while that of ovotransferrin appeared to be around 93% recovery rate and 80% purity. These protocols seem to be economical and practical for a large-scale purification of IgY and ovotransferrin from chicken egg because ethanol and resin used in the protocols can be regenerated as well as they are simple and rapid methods. The combination of 100 mM-NaHCO3 to ovotransferrin was found to be bacteriostatic against E. coli O157:H7 and L. monocytogenes in BHI broth. Also ovotransferrin solution added with 0.5% citric acid or Zn-bound ovotransferrin inhibited the growth E. coli O157:H7 and L. monocytogenes in BHI broth respectively. Also, EDTA and lysozyme promoted antibacterial activity of ovotransferrin solution combined with 100 mM-NaHCO3 against E. coli O157:H7 and L.monocytogenes respectively. Based on these results, this study claims that ovotransferrin combined citric acid, NaHCO3, and EDTA or lysozyme seemed to be useful antibacterial agents for controlling E. coli O157:H7 and L. monocytogenes.